Table 2.

Primer and probe sequences used to quantify gene expression by real-time PCR. Primer sequences for toll-like-receptor 2, 4, 6, 7 and 9 [17] and IL-4δ2 [18] have previously been published.

Genes	* Probe sequence 5'-(FAM-TAMRA)-3' ◇ L primer-5'-3' □ R primer-5'-3'	Product size
IL-4	* AAACCTTCTGCAGGGCTGCGAC	71 bp
	◇ GCTGCCTCCAAGAACACAAC
	□ CTGTAGAACTGCCGGAGCAC
HuPO	*TGCCAGTGTCTGTCTGCAGATTGG	105 bp
	◇ GCTTCCTGGAGGGTGTCC
	□ GGACTCGTTTGTACCCGTTG
NOD1	* CCTGGCTCCGACATCGGTGA	133 bp
	◇ AAGCGAAGAGCTGACCAAAT
	□ TCCCAGTTTAAGATGCGTGA
NOD2	* CCGAGGCATCTGCAAGCTCA	82 bp
	◇ CTGCAAGGCTCTGTATTTGC
	□ CTCGCAGTGAAGAGCACATT
TNF-α	* CAGCCACTGGAGCTGCCCCT	102 bp
	◇ AGCCCATGTTGTAGCAAACC
	□ GCTGGTTATCTCTCAGCTCCA
FLIP	* TGGATTGCTGCTTGGAGAACATTCC	114 bp
	◇ GTTCAAGGAGCAGGGACAAG
	□ ATCAGGACAATGGGCATAGG
FLICE	* ACTTGGATGCAGGGGCTTTGACCAC	102 bp
	◇ AAGTGCCCAAACTTCACAGC
	□ GGGGCTTGATCTCAAAATGA
Bax	* AAGTAGAAAAGGGCGACAACCCGGC	105 bp
	◇ GAGAGGTCTTTTTCCGAGTGG
	□ GCCTTGAGCACCAGTTTGCTG
Bfl-1	* CCACAACCTGGATCAGGTCCAAGCA	105 bp
	◇ GGCTGGCTCAGGACTATCTG
	□ TTTGGACTGAGAACGCAACA
Bcl-2	* CAAAGGCATCCCAGCCTCCGTTA	118 bp
	◇ AGTACCTGAACCGGCACCT
	□ TTCAGAGACAGCCAGGAGAAA