Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant property | Reference or origin |
| Escherichia coli | ||
| DH5α | F-, φ80dlacZΔM15 Δ(lacZYA-argF)U169 | Invitrogen |
| deoR recA1 endA1 hsdR17(rk-, mk+) | ||
| phoA supE44 λ- thi-1 gyrA96 relA1 | ||
| S17-1 | recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 | [45] |
| Pseudomonas aeruginosa | ||
| PAO1 | Wild type | [46] |
| PAO1ΔlasR | PAO1 derivative; ΔlasR::TcR | [47] |
| PAO1ΔrhlR | PAO1 derivative; ΔrhlR::GmR | [47] |
| PAO1ΔrhlRΔlasR | PAO1 derivative; ΔrhlR::GmR ΔlasR::TcR |
|
| [47] | ||
| PAO-rpoS | PAO1 derivative; rpoS::GmR | [15] |
| MSC100 | PAO1 derivative; ΔrhlRΔlasR, pBADlasR | This study |
| MSC110 | PAO1 derivative; ΔrhlRΔlasR, pBADrhlR | This study |
| MSC120 | PAO1 derivative; rpoS::GmR, pBADrpoS | This study |
| Plasmids | ||
| pFLP2 | Source of Flp recombinase; ApR | [37] |
| pJN105 | araC-pBAD cloned in pBBR1MCS-5; GmR | [41] |
| pJN105.lasR | lasR in pJN105 | [48] |
| pJN105.rhlR | rhlR in pJN105 | This study |
| pJN105.rpoS | rpoS in pJN105 | This study |
| pSW196 | mini-CTX1 based plasmid containing | [40] |
| araC-pBAD from pBAD30; TcR | ||
| pSW196.lasR | lasR in pSW196 | This study |
| pSW196.rhlR | rhlR in pSW196 | This study |
| pSW196.rpoS | rpoS in pSW196 | This study |