Table 1.

CheY92KR does not support the Acs-mediated CW response to acetate

Relevant genotype*	Mean flagellar rotational bias ± SEM (n)†
	Wild-type CheY‡		CheY92KR
	Prestimulus	+Acetate	Prestimulus	+Acetate
Cells grown with acetate
1. Δ(cheA-cheZ) ace+	0.93  ±  0.04 (30)	0.04  ±  0.03 (31)	0.93  ±  0.04 (28)	0.14  ±  0.05 (30)
2. Δ(cheA-cheZ) Δ(ackA pta)	0.83  ±  0.06 (29)	0.27  ±  0.07 (29)	0.82  ±  0.05 (30)	0.75  ±  0.07 (30)
3. Δ(cheA-cheZ) Δ(ackA pta) Δacs	0.69  ±  0.11 (15)	0.68  ±  0.16 (8)	0.89  ±  0.08 (12)	0.78  ±  0.20 (5)
Cells grown without acetate
4. Δ(cheA-cheZ) ace+	0.44  ±  0.06 (39)	0.00  ±  0.00 (32)	0.99  ±  0.00 (30)	0.03  ±  0.03 (30)
5. Δ(cheA-cheZ) Δ(ackA pta)	0.96  ±  0.03 (21)	0.99  ±  0.01 (31)	1.00  ±  0.00 (20)	1.00  ±  0.00 (14)
6. Δ(cheA-cheZ) Δ(ackA pta) Δacs	0.77  ±  0.08 (25)	0.72  ±  0.14 (11)	0.92  ±  0.07 (14)	0.90  ±  0.06 (16)

^*Host strains used were RBB1106, RBB1097, and RBB1109. 

^†Bias, fraction of time spent in CCW rotation; n, number of cells observed. 

^‡CheY proteins expressed from pRL22ΔZ.