Figure 2.

Correction of aberrant splicing by U7 snRNA. Total RNA from HeLa IVS2–705 cell line transiently transfected with 0.5, 1, and 2 μg of U7 snRNA constructs (shown at the top) was analyzed by RT-PCR. Lanes: 1, untreated IVS2–705 cells; 2–4, cells transfected with U7SmOPT; 5–7 and 8–10, cells transfected with U7.5 and U7.3 constructs, respectively; 11, RNA from human blood. The sizes (in nucleotides) of PCR bands representing aberrantly and correctly spliced mRNAs are indicated on the left. The results of RT-PCR are also shown in Figs. 3, 4A, 5, 6A, and 7B. Quantification of these results took into account the approximately 2-fold higher [³²P]dAp content of the aberrant PCR product than that of the correct one.