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. 1998 Apr 28;95(9):4929–4934. doi: 10.1073/pnas.95.9.4929

Figure 6.

Figure 6

(A) Correction of splicing in cell lines stably expressing U7.324 snRNA. The IVS2–705 cell line was cotransfected with the U7.324 construct and a plasmid carrying the hygromycin-resistance gene. Total RNA from the resulting hygromycin-resistant cell lines was analyzed by RT-PCR. Lanes: 1, RNA from human blood; 2, RNA from IVS2–705 cell line; 3–5, stable correction of splicing in three independent cell lines (705U7.324.1, .4, and .9) as indicated at the top of the figure. (B) Correlation of splicing correction with stable expression of U7.324 snRNA. U7.3 plasmid (200 ng, lane 1), total DNA (lanes 2–5), or total RNA (lanes 6–13) from the cell lines shown in Fig. 6A were analyzed by PCR and RT-PCR, respectively, by using U7-specific primers (see Fig. 1B and Materials and Methods). Because isolated RNA possibly could be contaminated with genomic DNA, in lanes 10–13 the RNA was analyzed with the reverse transcription step omitted in the assay.