Figure 3.

Activation of the wild-type and mutant LDL receptor promoter by USF2-VP16. (A). HepG2 cells were transfected with increasing amounts of plasmid cytomegalovirus-USF2-VP16 expression vector (abscissa) and wild-type (Wt; •) or mutant (LDLE; ▪) LDL receptor promoter luciferase reporter plasmids (5 μg per 60-mm dish) along with 5 μg per 60-mm dish of cytomegalovirus-β-galactosidase plasmid as indicated. (B) Companion dishes were transfected with the same reporter plasmids alone (−) or along with the SREBP-1a expression vector (+) as indicated (30 ng per 60-mm dish). Transfections were performed and analyzed as described in Materials and Methods and in the legend to Fig. 2.