Figure 4.

Evaluation of sterol regulation for the wild-type and mutant LDL receptor promoters. The wild-type (Wt) or mutant (LDLE) LDL receptor promoter fused to the luciferase gene were transfected into CV-1 cells (5 μg per 60-mm dish) and 18 hr after transfection dishes were fed either medium containing lipoprotein-depleted serum (Induced) or medium containing lipoprotein-depleted serum plus 10 μg/ml of cholesterol and 1 μg/ml of 25-hydroxycholesterol (Suppressed). The cells were cultured for an additional 24 hr before harvest. The samples were processed as described in Materials and Methods. Each value represents the average of two independent experiments each performed in duplicate.