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Binding of sauvagine to membranes of HEK 293 cells transfected with hCRF-R1, h1(1–123)x1(124–415), h1(1–92)x1(93–415), and h1(1–67)x1(68–415) (A) or xCRF-R1, x1(1–123)h1(124–415), x1(1–92)h1(93–415), and x1(1–67)h1(68–415) (B). Competitive binding was performed with 125I-labeled h/rCRF and increasing concentrations (0.01 nM to 1 μM) of unlabeled sauvagine. Data represent duplicates from one representative experiment repeated at least three times.
