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Stimulation of intracellular cAMP accumulation of HEK 293 cells stably transfected with hCRF-R1, h1(1–123)x1(124–415), h1(1–92)x1(93–415), and h1(1–67)x1(68–415) (A) or xCRF-R1, x1(1–123)h1(124–415), x1(1–92)h1(93–415), and x1(1–67)h1(68–415) (B) by sauvagine. Cells were incubated with increasing concentrations of sauvagine for 30 min at 37°C, and cAMP was determined. The results are representatives of three stimulations.
