Table 1.

Binding properties of hCRF-R1, xCRF-RI, and six chimeric receptors

Receptor	h/rCRF Kd, nM	oCRF Kd, nM	Sauvagine Kd, nM
hCRF-R1a	2.2 ± 1.2	2.6 ± 1.1	2.1 ± 1.1
h1(1–123)x1(124–415)	1.8 ± 1.2	1.9 ± 0.3	1.6 ± 1.0
h1(1–92)x1(93–415)	1.6 ± 0.7	2.9 ± 1.5	2.2 ± 1.2
h1(1–67)x1(68–415)	2.5 ± 1.0	30.2 ± 2.6*	36.9 ± 4.6*
x1(1–67)h1(68–415)	2.4 ± 1.1	3.4 ± 2.0	2.1 ± 1.3
x1(1–92)h1(93–415)	3.0 ± 1.4	31.2 ± 4.6*	43.9 ± 4.2*§
x1(1–123)h1(124–415)	4.4 ± 2.1	36.3 ± 3.9†	61.4 ± 9.2*§
xCRF-R1	3.1 ± 1.4	31.7 ± 2.6†	51.4 ± 6.6*‡

Data are the mean ± SEM of three binding studies. Statistically significant differences: 

^*P < 0.0001 vs. h/rCRF. 

^†P < 0.001 vs. h/rCRF 

^‡P < 0.001 vs. oCRF. 

^§P < 0.01 vs. oCRF. 

^a From ref. 28.