Table 2.

Stimulation of cAMP accumulation by different CRF-like peptides in HEK 293 cells expressing hCRF-R1, xCRF-R1, or six different chimeric receptor cDNAs

Receptor	h/rCRF EC50, nM	oCRF EC50, nM	Sauvagine EC50, nM
hCRF-R1a	1.6 ± 0.2	1.8 ± 0.2	0.9 ± 0.1†¶
h1(1–123)x1(124–415)	0.8 ± 0.2	1.2 ± 0.1	0.4 ± 0.1‡∥
h1(1–92)x1(93–415)	0.9 ± 0.1	1.3 ± 0.2	1.1 ± 0.2
h1(1–67)x1(68–415)	0.7 ± 0.2	4.1 ± 0.3*	20.9 ± 0.5*§
x1(1–67)h1(68–415)	0.8 ± 0.2	1.4 ± 0.3	1.0 ± 0.2
x1(1–92)h1(93–415)	0.7 ± 0.1	4.9 ± 0.7*	20.9 ± 0.6*§
x1(1–123)h1(124–415)	0.8 ± 0.2	6.5 ± 0.9*	26.1 ± 0.7*§
xCRF-R1	1.1 ± 0.1	7.1 ± 0.8*	39.7 ± 0.8*§

Data are the mean ± SEM of three stimulations. Statistically significant differences: 

^*P < 0.0001 vs. h/rCRF. 

^†P < 0.01 vs. h/rCRF 

^‡P < 0.05 vs. h/rCRF. 

^§P < 0.0001 vs. oCRF. 

^¶P < 0.001 vs. oCRF. 

∥ P < 0.01 vs. oCRF. 

^a From ref. 28.