Table 3.

Binding properties of different mutants of hCRF-R1 or xCRF-R1

Receptor	h/rCRF Kd, nM	oCRF Kd, nM	Sauvagine Kd, nM
h1x1(Q76,G81,V83,H88,L89)	2.6 ± 1.3	31.9 ± 4.1*	34.9 ± 4.7*
h1x1(H88,L89)	1.8 ± 0.8	5.2 ± 1.4	4.5 ± 2.1
h1x1(Q76,G81,V83)	2.5 ± 1.5	28.4 ± 2.0*	25.6 ± 4.8*
h1x1(Q76,G81)	1.4 ± 0.9	2.4 ± 0.4	1.5 ± 0.5
h1x1(G81,V83)	3.5 ± 1.9	4.4 ± 2.4	3.9 ± 1.5
x1h1(R76,N81,G83,L88,A89)	3.8 ± 1.2	4.4 ± 2.3	4.5 ± 2.7
x1h1(L88,A89)	1.3 ± 0.3	12.6 ± 3.5†	11.2 ± 2.4†

Data are the mean ± SEM of three binding studies. Statistically significant differences: 

^*P < 0.001 vs. h/rCRF. 

^†P < 0.01 vs. h/rCRF.