Table 4.

Stimulation of cAMP accumulation of different CRF-like peptides in HEK 293 cells expressing different mutants of hCRF-R1 or xCRF-R1 cDNAs

Receptor	h/rCRF EC50, nM	oCRF EC50, nM	Sauvagine EC50, nM
h1x1(Q76,G81,V83,H88,L89)	0.7 ± 0.2	4.9 ± 0.4*	20.4 ± 0.8*†
h1x1(H88,L89)	1.0 ± 0.1	2.8 ± 0.2*	2.6 ± 0.3*
h1x1(Q76,G81,V83)	0.9 ± 0.2	8.3 ± 1.0*	10.1 ± 0.7*
h1x1(Q76,G81)	1.1 ± 0.2	1.4 ± 0.3	1.3 ± 0.3
h1x1(G81,V83)	2.2 ± 0.2	2.7 ± 0.3	2.4 ± 0.3
x1h1(R76,N81,G83,L88,A89)	1.0 ± 0.2	1.4 ± 0.2	1.2 ± 0.1
x1h1(L88,A89)	1.2 ± 0.2	10.3 ± 0.5*	10.2 ± 0.9*

Data are the mean ± SEM of three stimulations. Statistically significant differences: 

^*P < 0.0001 vs. h/rCRF. 

^†P < 0.0001 vs. oCRF.