Figure 1.

SWI/SNF complex binding to nucleosome cores or naked DNA. Purified SWI/SNF complex was incubated with a 176-bp 5S RNA gene probe either as reconstituted nucleosome cores (lanes 1–8) or as naked DNA (lanes 9–16). SWI/SNF was present at 0 nM in lanes 1 and 9, 3 nM in lanes 2 and 10, 10 nM in lanes 3 and 11, and 30 nM in lanes 4–8 and 12–16. Distamycin A was present during SWI/SNF binding in lanes 5 and 13 (0.1 μM), lanes 6 and 14 (1 μM), lanes 7 and 15 (10 μM), and lanes 8 and 16 (100 μM). All lanes contain 9 nM total nucleosomal or naked DNA (mainly HeLa unlabeled oligonucleosomes or DNA) and 1 mM Mg-ATP. Samples were run on 4% acrylamide gel (80:1, acrylamide/bis) in a Tris/glycine/EDTA buffer system.