Figure 4.

Perturbation of nucleosome core structure by SWI/SNF is stable for several hours after its detachment but eventually reverts back. Time-course experiment on the stability of the altered nucleosomal state induced by SWI/SNF. 5S nucleosome cores were incubated with 15 nM SWI/SNF complex for 30 min at 30°C in the presence of 1 mM ATP. Apyrase and 20-fold excess of both unlabeled HeLa oligonucleosomes and calf thymus DNA then were added. Reactions were put at 37°C for the indicated time before DNase I addition.