Figure 6.

Measurement of the intracellular level of Rsd. E. coli W3110 type A strain (19) was grown in LB medium at 37°C. Growth was monitored with a Klett–Summerson photometer. At the times indicated, cell lysates were prepared according to the method described previously (8, 9). The protein concentration was determined by using a protein assay kit (Bio-Rad). Aliquots containing 10 μg of total protein were subjected to the quantitative Western blot assay as employed previously (8, 9) by using the ECL reagent system (Amersham) for detection of the membrane-bound anti-Rsd antibodies.