Table 2.
The total and leaflet phospholipid compositions of the erythrocyte membrane from various mammalian species
| Phospholipid | Monkey
|
Horse
|
Rat
|
Mouse
|
Rabbit
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total | OL | IL | Total | OL | IL | Total | OL | IL | Total | OL | IL | Total | OL | IL | |
| CPs | 56.5 (55.6) | 89.4 (88.9) | 23.1 (22.2) | 56.3 (55.6) | 79.7 (77.8) | 33.2 (33.3) | 59.0 (61.0) | 87.8 (88.9) | 31.3 (33.3) | 60.8 (61.0) | 86.3 (88.9) | 37.8 (33.3) | 57.3 (55.6) | 87.8 (88.9) | 26.1 (22.2) |
| APs | 15.9 (16.7) | 0.0 (0.0) | 33.9 (33.3) | 15.7 (16.7) | 0.0 (0.0) | 31.2 (33.3) | 16.0 (16.7) | 2.0 (0.0) | 29.5 (33.3) | 14.9 (16.7) | 3.5 (0.0) | 25.2 (22.2) | 8.4 (11.1) | 0.0 (0.0) | 16.9 (22.2) |
| PE | 27.6 (27.8) | 10.6 (11.1) | 43.0 (44.4) | 28.0 (27.8) | 20.3 (22.2) | 35.6 (33.3) | 25.0 (22.2) | 10.2 (11.1) | 39.2 (33.3) | 24.3 (22.2) | 10.2 (11.1) | 37.0 (44.4) | 34.4 (33.3) | 12.2 (11.1) | 56.9 (55.6) |
| Phospholipid in leaflet, % | 50.2 | 49.8 | 49.7 | 50.3 | 49.0 | 51.0 | 47.5 | 52.5 | 50.5 | 49.5 | |||||
The inner and outer leaflet compositions were calculated based on published overall compositions and transbilayer distribution data (20–23). The unit is mol %. Standard deviations are, when given, similar to those in Table 1. When a range was given for the fraction of total lipid in the outer leaflet, the upper limit was chosen because this brings the transbilayer distribution of total phospholipids closer to the expected and generally observed 50:50 distribution. Incomplete digestion of the outer leaflet lipids is a common problem in the determination of transbilayer distributions (24). Lysophosphatidylcholine, which represents about 5, 4, and 2.5% of rat, mouse, and monkey erythrocyte phospholipids, respectively, was assumed to be present exclusively in the outer leaflet (see legend of Table 1). IL, inner leaflet; OL, outer leaflet.