Figure 4.
Effects of Bcl-XL, CsA, and zVAD-fmk on Bax-induced release of Cyt c from mitochondria. Mitochondria were treated (sometimes in duplicate) with 1 μM Bax or diluent control (C), with or without other reagents, and Cyt c release into supernatants was measured 1 hr later by immunoblotting. In A, 12 μM Bcl-XL was added to some samples simultaneously with Bax, as indicated. In B, 10 μM CsA or 10 μM zVAD-fmk was added 5 min prior to Bax. In C, the mitochondrial pellets were analyzed in addition to the supernatants (25 μg). Bax binding to mitochondria was determined by SDS/PAGE immunoblot analysis by using an anti-Bax antibody.