Table 3.
Search of differences in biologically significant sites between chaetognath ribosomal protein isoforms using Prosite
| Protein name | Isoform number | Aa numbers | EST numbers | % identity/overall similarity | Motifs which are different |
| SSU ribosomal proteins | |||||
| S7 | 1 | 194 | 30 | 82.0/97.9 | cAMP:1 – PKC:3 |
| 2 | 194 | 1 | cAMP:2 – PKC:6 | ||
| S8 | 1 | 208 | 9 | is.1/is.2: 96.6/100 | amidation:2 – cAMP:2 – myristil:2 – nuclear:3 – PKC:6 |
| 2 | 208 | 8 | is.2/is.3: 97.1/100 | amidation:1 – cAMP:4 – myristil:2 – nuclear:4 – PKC:8 | |
| 3 | 208 | 2 | is.1/is.3: 97.1/100 | amidation:2 – cAMP:4 – myristil:1 – nuclear:4 – PKC:7 | |
| S11 | 1 | 156 | 24 | 98.1/99.4 | nuclear:1 |
| 2 | 156 | 1 | nuclear:0 | ||
| S15 | 1 | 144 | 7 | 99.3/100 | N.D. |
| 2 | 144 | 8 | N.D. | ||
| S16 | 1 | 146 | 9 | 93.8/96.6 | CK2:2 |
| 2 | 145 | 13 | CK2:3 | ||
| S17 | 1 | 134 | 19 | 87.4/96.3 | CK2:0 – myristil:0 – nuclear:1 – sulfation:1 |
| 2 | 135 | 10 | CK2:1 – myristil:1 – nuclear:0 – sulfation:0 | ||
| S21 | 1 | 81 | 2 | 83.1/92.8 | cAMP:1 – myristyl:2 – PKC:3 – tyr:0 |
| 2 | 83 | 10 | cAMP:2 – myristyl:1 – PKC:2 – tyr:1 | ||
| S25 | 1 | 115 | 14 | is.1/is.2: 82.8/93.9 | cAMP:1 – CK2:0 – myristil:2 – nuclear:1 |
| 2 | 114 | 2 | is.2/is.3: 71.7/83.5 | cAMP:1 – CK2:1 – myristil:1 – nuclear:1 | |
| 3 | 127 | 1 | is.1/is.3: 73.2/83.5 | cAMP:0 – CK2:0 – myristil:2 – nuclear:0 | |
| S28 | 1 | 64 | 8 | 98.4/100 | N.D. |
| 2 | 64 | 14 | N.D. | ||
| LSU ribosomal proteins | |||||
| L7 | 1 | 245 | 13 | 85.7/95.5 | cAMP:1 – PKC:2 |
| 2 | 243 | 23 | cAMP:0 – PKC:3 | ||
| L15 | 1 | 206 | 1 | 98.1/99.0 | N.D. |
| 2 | 207 | 1 | N.D. | ||
| L21 | 1 | 161 | 5 | 97.5/88.1 | myristil:2 – PKC:4 |
| 2 | 161 | 2 | myristil:1 – PKC:5 | ||
| L22 | 1 | 121 | 10 | 81.2/92.1 | amidation:1 - CK2:2- myristil:4 |
| 2 | 128 | 1 | amidation:0 - CK2:1- myristil:2 | ||
| L27 | 1 | 136 | 15 | 88.2/99.2 | myristil:0 – PKC:2 |
| 2 | 136 | 26 | myristil:1 – PKC:3 | ||
| L27a | 1 | 145 | 3 | 89.6/97.2 | CK2:1 |
| 2 | 145 | 10 | CK2:0 | ||
| L30 | 1 | 114 | 20 | 96.5/98.2 | PKC:4 |
| 2 | 114 | 6 | PKC:5 | ||
| L34 | 1 | 130 | 16 | 95.4/97.7 | cAMP:3 – PKC:1 |
| 2 | 131 | 8 | cAMP:4 – PKC:2 | ||
| L35 | 1 | 123 | 13 | 89.4/98.4 | tyr:0 |
| 2 | 123 | 11 | tyr:1 | ||
| L36 | 1 | 105 | 16 | 90.6/98.1 | PKC:4 |
| 2 | 106 | 32 | PKC:2 | ||
| L37 | 1 | 100 | 9 | 99.0/99.0 | N.D. |
| 2 | 100 | 8 | N.D. | ||
| L38 | 1 | 70 | 29 | 92.9/100 | asn:1 – CK2:1 – PKC:3 |
| 2 | 70 | 5 | asn:0 – CK2:0 – PKC:2 | ||
| L39 | 1 | 51 | 13 | is.1/is.2: 96.1/98.0 | myristil:0 |
| 2 | 51 | 1 | is.1/is.3: 98.0/98.0 | myristil:1 | |
| 3 | 51 | 1 | is.2/is.3: 94.1/96.1 | myristil:0 | |
Within a protein family, each of the isoforms are putatively encoded by different types of cDNA, except the L39 isoform-1 and isoform-3 which are encoded by cDNAs belonging to two different subtypes (TTT and TAC respectively) of a same type. Abbreviations: amidation, amidation site; cAMP, cAMP- and cGMP-dependent protein kinase phosphorylation site; CK, casein kinase II phosphorylation site; myristil, N-myristoylation site ; N.D., no difference; nuclear, bipartite nuclear targeting sequence; PKC, protein kinase C phosphorylation site ; tyr, tyrosine sulfation site.