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Clustering of β1a in dysgenic myotubes depends on coexpression of α1S with intact β interaction domain. βGFP is expressed alone (a) or in combination with either the wild-type α1S (b), the α1S-Y366S (c), or the α1S-Δ351–380 mutant (d). The β1a subunit forms clusters only when coexpressed with the wild-type α1S subunit (b). When coexpressed with the mutated α1S subunits the distribution of βGFP is diffuse, as if expressed alone (compare c and d with a). N, myotube nuclei. (Bar, 20 μm.)
