Figure 1. Comparison of pl1 Expression between rmr1 Mutants and Heterozygous Siblings.

pl1 RNA levels increase significantly in rmr1 mutants, while transcription rates of paramutant alleles are unaffected.

(A) The relative mean transcription rates from four independent sets of +/rmr1–3 (open) and rmr1–3/rmr1–3 (filled) siblings (± standard error of the mean) at the indicated loci.

(B) RNase protection analysis comparing pl1 and actin1 RNA levels in the same individual plants and tissues used for in vitro transcription analysis.

(C) Quantification of relative pl1 RNA levels from analyses as represented in (B).