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Cell proliferation. p70s6k+/+ ES cells or p70s6k−/− ES cells were incubated in DMEM medium supplemented with 15% FCS and leukemia inhibitory factor (1,000 units/ml), in the presence or absence of rapamycin (10 ng/ml) for 72 hr. Cells were counted by using a Coulter counter (Hialeah, FL).
