Figure 4.

Adrenergic receptor distribution in CD117⁺ progenitors. Murine bone marrow cells were enriched for CD117⁺ using Ab coupled micro-bead technology and labeled with primary antibody specific for adrenergic receptor subtypes with and without specific blocking peptides followed by FITC conjugated secondary antibody. Confocal images were obtained in a Zeiss LSM 510 confocal microscope. Green color represents binding of the polyclonal Abs to the C-terminus of adrenergic receptor subtypes in CD117⁺ cells. Image resolution=1024 × 1024; Scale bar=10μm. Histograms were generated by FACS. Logarithmic scale of mean fluorescent intensity of specific adrenergic receptor subtype is represented on the X-axis and the percentage of cells expressing α₁-, α₂- or β₂- adrenergic receptors is represented on the Y-axis. Panels A, B and C represents α₁-, α₂- and β₂- adrenergic receptors respectively.