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. Author manuscript; available in PMC: 2008 Apr 30.
Published in final edited form as: J Neuroimmunol. 2007 Apr 10;186(1-2):27–36. doi: 10.1016/j.jneuroim.2007.02.007

Figure 6.

Figure 6

LPS mediated TNF-α response of MØ differentiated from bone marrow myeloid progenitors with and without adrenergic stimulation during their development. Bone marrow ER-MP20+, ER-MP12+, CD34+, CD117+ and HSCs were allowed to differentiate into mature MØ in vitro with specific growth factor cocktail with and without isoproterenol, phenylephrine and clonidine added on day 0. At the end of culture (7–10 days), the adherent MØ were washed, stimulated with LPS (100ng/ml for 18h) and the conditioned medium was collected for TNF-α measurements (ELISA). Data represents soluble TNF-α in the conditioned medium normalized to protein content and expressed as a percentage in comparison to vehicle treatment taken as 100%.