Table 1.
Hematopoietic Progenitors | α1- AR# | α1- AR+ Blocking peptide# | Mean Fold Difference | α2- AR# | α2- AR+ Blocking peptide# | Mean Fold Difference | β2- AR# | β2- AR+ Blocking peptide | Mean Fold Difference |
---|---|---|---|---|---|---|---|---|---|
ER-MP-20+ | 5,094±194 | 2,088±40 | 2.44 | 10,431±809 | 4,804±96 | 2.17 | 28,254±1,805 | 8,542±870 | 3.31 |
ER-MP-12+ | 1,477±47 | 756±13 | 1.95 | 7,745±155 | 3,769±120 | 2.05 | 11,120±987 | 4,356±67 | 2.55 |
CD34+ | 1,645±38 | 908±25 | 1.81 | 7,654±167 | 3,356±87 | 2.28 | 4,648±99 | 1,644±49 | 2.83 |
CD117+ | 1,542±29 | 739±16 | 2.09 | 1,389±46 | 817±34 | 1.70 | 5,908±128 | 2,104±32 | 2.81 |
MFI (mean fluorescent intensity) expressed as Mean±SEM. Approximately a two-fold difference in MFI of the bound specific primary antibodies in the presence and absence of competing blocking peptides signifies the specificity of the α1-, α2- and β2- adrenergic receptor antibodies.