Table 2.

Effect of Tween-20 concentrations on recovery of colony-forming units from climax biofilms and stationary-phase planktonic cultures

	Tween-20 concentrations (%)
	0.00	0.10	0.25	0.50	1.00	2.00
Climax biofilms1
Biofilm 1	1.6 × 1011	5.8 × 1010	6.0 × 109	4.3 × 109	4.9 × 109	4.6 × 109
Biofilm 2	1.3 × 1011	4.5 × 1010	5.0 × 109	6.3 × 109	5.7 × 109	5.9 × 109
Biofilm 3	1.5 × 1011	1.5 × 1010	1.4 × 1010	8.9 × 109	8.8 × 109	8.6 × 109
Biofilm 4	1.5 × 1011	1.5 × 1010	2.0 × 109	3.3 × 109	4.3 × 109	3.3 × 109
Biofilm 5	4.2 × 1011	4.2 × 1010	8.2 × 109	6.6 × 109	3.3 × 109	4.9 × 109
Biofilm 6	1.6 × 1011	7.7 × 1010	1.2 × 1010	8.9 × 109	6.6 × 109	6.9 × 109
Biofilm 7	2.9 × 1011	9.3 × 1010	2.9 × 109	3.6 × 109	4.5 × 109	3.7 × 109
Biofilm 8	4.3 × 1011	1.5 × 1010	1.2 × 1010	9.6 × 109	5.6 × 109	4.8 × 109
Planktonic cultures2
A. odontolyticus	8.0 × 107	1.2 × 108	1.3 × 108	1.5 × 108	8.0 × 107	9.6 × 107
S. gordonii	1.0 × 108	2.0 × 108	2.0 × 108	1.2 × 108	2.0 × 108	1.4 × 108
S. parasanguis	2.2 × 108	2.0 × 108	2.1 × 108	2.0 × 108	2.4 × 108	2.0 × 108
F. nucleatum	4.3 × 107	4.5 × 107	3.5 × 107	4.3 × 107	3.6 × 107	3.9 × 106
T. forsythensis	4.3 × 104	4.2 × 104	3.5 × 104	4.6 × 104	3.6 × 104	8.0 × 103
P. gingivalis	3.5 × 106	4.2 × 106	2.6 × 106	2.9 × 106	5.6 × 105	4.6 × 105
P. intermedia	2.7 × 107	3.6 × 107	2.6 × 108	4.5 × 107	2.6 × 107	5.3 × 105

¹Single determination.

²Mean of three determinations.