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. Author manuscript; available in PMC: 2007 Oct 15.
Published in final edited form as: Mol Cell. 2007 Feb 23;25(4):531–542. doi: 10.1016/j.molcel.2007.01.015

Figure 5.

Figure 5

Enzyme dependent production of AMP and non-enzyme dependent hydrolysis of histidyl adenylate at pH 7.5 and 37°C.

(A) Thin layer chromatography experiment showing that the production of AMP is strictly dependent on the presence of enzyme over the relevant time course of experimentation. The left side of the panel is the complete reaction including enzyme, while the right panel is the complete reaction in the absence of enzyme. The reactions were prepared, imaged, and analyzed as described in “Experimental Procedures”.

(B) TLC showing the accumulation and destruction of the adenylate fraction during the experimental time course in the absence of tRNA. The observed adenylate is only a small fraction of the total AMP production.

(C) Graphical re-plot of data in (B), showing accumulation of adenylate in solution, and its subsequent decomposition in solution.