TABLE 3.
Binding of pro-HP14 to microbes or their surface polysaccharides
| Curdlan | S. cerevisiae | Zymosan | M. luteus | E. coli | Peptidoglycan | ||
|---|---|---|---|---|---|---|---|
| % | |||||||
| Pro-HP14 in plasmaa | 0 | 25 | 0 | 66 | 100 | 33 | |
| Purified pro-HP14b | 82 | 97 | 97 | 94 | 95 | Not determined |
Binding of pro-HP14 in plasma is represented as % of the total proenzyme in the supernatant. The relative levels of pro-HP14 were determined by immunoblotting and densitometry of the protein band.
Binding of pro-HP14 to microbial cells or cell-wall components is represented as % of the total purified zymogen in the unbound fraction.