Figure 2.

Nucleotide sequence and structural features of the Manduca sexta PAP-3 gene. Nucleotides in the 5′ flanking region are assigned negative numbers. Nucleotide 1 is assigned based on the primer extension results (Fig. 3). Exon sequences are underlined with the encoded amino acid sequences listed below translated exons, using a one-letter code under the second nucleotide of each codon. While some regions of the intron sequences (marked ‘–’) are not shown, their sizes and positions are indicated. GATA boxes (6-nucleotide) and ISRE sites (13-nucleotide), bold and double underlined; NF-κB motifs (10-nucleotide) and EcRE (15-nucleotide), bold and single underlined; TATA box (6-nucleotide), bold italic. The mismatches in these motifs are in lower case. Sequences of the 3′ end of λ7 (4.32 kb), gap, and the 5′ end of λ3 (4.51 kb) are not shown. The SacI and EcoRI recognition sites (in bold) are numbered consecutively as 2336–2341 and 2342–2347, respectively. Single nucleotide polymorphic sites are in bold italic on the DNA sequence. Among them, nonsynonymous substitutions are further indicated on the affected amino acid residues (bold and underlined). The Cys residues in the clip domain and the catalytic residues in the SP domain are indicated with ‘+’ and ‘#’, respectively.