Figure 5.
qRT-PCR results for CYP1A1 and CYP1B1 where the DRE activity appears to be greater than the ARE activity based on the activation of these genes by TCDD (DRE activator) and TBHQ (ARE activator). MCF-10A cells were treated with DMSO (solvent control), TCDD (10 nm), TBHQ (20 μM), 10 μg/ml EGF, 1 μM 1,6-BPQ, or 1 μM 3,6-BPQ for 18 hrs, followed by extraction of RNA and Taqman analysis. Results shown are the Mean ± SD for triplicate determination using Taqman probes (p < .01).