TABLE III.
Analysis of Gene Promoters Induced by 1,6- and 3,6-B[a]P Quinones*
| Gene Promoter | Number of Response Elements in Promoter | Response Element Location$ | ||
|---|---|---|---|---|
| ARE/EpRE1 | DRE/XRE2 | ARE/EpRE1 | DRE/XRE2 | |
| HMOX1# | 2 | 5 | 2411, 7119 | 2831, 4164, 6153, 7133, 7365 |
| CYP1A1# | 1 | 13 | 7529 | 36, 379, 482, 873, 877, 965,1038,1364, 2099, 3058, 5594, 7497, 9171 |
| CYP1B1# | 0 | 7 | ni3 | 825, 844, 980, 1015, 1483, 2304, 8531 |
| AKR1C1# | 1 | 5 | 1054 | 1100, 2590,2648, 3698, 8551 |
| ALDH3A1# | 3 | 10 | 4930, 5174, 5660 | 74, 746, 1182, 2122, 2550, 4220, 4272, 5037, 7968, 8417 |
| NQO1# | 1 | 7 | 381 | 306, 3119, 5031, 5556, 7330, 7644, 9516 |
| EPHX1# | 0 | 8 | ni | 1525, 2410, 4379, 6141, 7598, 7685, 9900 |
| AKR1B10 | 2 | 6 | 273, 7701 | 924, 1823, 5027, 5351, 6392, 9093 |
| AKR1C2 | 3 | 0 | 2411, 5028, 7462 | ni |
| SLC7A11 | 0 | 3 | ni | 4795, 7566, 7605 |
| GCLC | 0 | 5 | ni | 228, 2897, 3391, 3989, 5568 |
| GCLM | 3 | 1 | 2217, 3368, 9277 | 1966 |
| PIR | 0 | 6 | ni | 46, 78, 867, 2235, 4092, 9553 |
| ASPH | 1 | 4 | 3214 | 117, 405, 7716, 7838 |
| POGZ | 2 | 10 | 3868, 8575 | 541, 1669, 2024, 4578, 5155, 7101, 7393, 7587, 8859, 8954 |
| AHR | 1 | 1 | 4230 | 2598 |
| EGFR | 2 | 1 | 173, 7005 | 5095 |
| SQSTM1 | 3 | 11 | 1901, 4087, 9936 | 1271, 1533, 1694, 1931, 2059, 2173, 3255, 3841, 5025, 6878, 9249 |
| ING1 | 3 | 13 | 468, 1605, 7732 | 169, 1135, 1388, 2190, 2409, 2499, 3606, 3616, 3846, 5231, 6201, 9720, 9972 |
| IER3 | 3 | 9 | 2878, 3637, 7204 | 431, 762, 1585, 2679, 4096, 7929, 8382, 9387, 9397 |
| VIPR1 | 0 | 3 | ni | 796, 4453, 5503 |
| UGT1A9 | 3 | 4 | 930, 4537, 9545 | 2205, 2381, 4283, 6051 |
| NQO2 | 0 | 7 | ni | 701, 3047, 3724, 5329, 7439, 9588, 9600 |
*
Promoter analysis was performed using MatInspector (Cartharius et al., 2005) for 10Kb upstream of the gene transcriptional initiation site.
$
Listed as start of response element location upstream of transcriptional initiation site.
#
Expression level determined by both qPCR and microarray.
1
Antioxidant response element (ARE) = the nuclear factor (erythroid-derived 2)-like 2, NRF2 site.
2
Dioxin response element (DRE) or Xenobiotic response elements (XRE) = includes the aryl hydrocarbon/ dioxin receptor, aryl hydrocarbon receptor/ arnt heterodimers, and aryl hydrocarbon/ arnt heterodimers, fixed core sites.
3
None identified.