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. Author manuscript; available in PMC: 2008 Jul 15.
Published in final edited form as: Dev Biol. 2007 May 3;307(2):356–367. doi: 10.1016/j.ydbio.2007.04.040

Figure 2.

Figure 2

(A-F) Whole mount in situ hybridization analysis of the Mis expression in the E13.5 embryonic male (A), XX sex-reversed (B and E), XX Fog2 heterozygous (C and F) and XX (female) (D) gonads. Mis expression is absent from the control sample in (D); it is also not observed in the heterozygous Fog2 transgenic (Ods or Wt1-Sox9) gonads (C, F). (G) Semi-quantitative RT-PCR analysis of Mis gene expression in the XY (1), XX (2), XX Wt1-Sox9 (3) and XX Wt1-Sox9 Fog2+/− (4) E12.5 gonads. The positions of the bands corresponding to the Mis (arrow) and Hprt control (double arrow) PCR fragments are shown. (H) Whole mount in situ hybridization analysis of the Mis expression in the E14.0 double transgenic XX Wt1-Sox9/Wt1-Sox9 Fog2+/− gonad where robust Mis expression is apparent.