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(A-G) Whole mount in situ hybridization analysis of the Sox9 expression in the XY (A-C) and XX (D-G) E12.0 gonads. Sox9 expression is detectable in the male control and Ods sex-reversed gonads (A, D, E); Sox9 expression is absent in the GATA4ki/ki homozygous gonads (B-C; F-G) even in the Ods mutants (XY, C; XX, G). (H-I) Whole mount in situ hybridization analysis of the Sox9 expression in the E13.5 XY (H) and XX (I) Wt1-Sox9 Fog2−/− gonads; Sox9 expression is absent.
