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Kinetic parameters of the wild type and three pyrimethamine-resistant DHFR enzymes. Values are the average of at least 7 experiments ± the standard deviation.
| Genotype | kcat (sec−1) | KmDHF (μM) | Km NADPH (μM) | kcat/Km |
|---|---|---|---|---|
| WILD TYPE | 1.24 ± 0.11 | 44.4 ± 12.1 | 23.2 ± 9.5 | 0.028 |
| 51I/59R/108N | 1.23 ± 0.35 | 20.9 ± 5.5 | 37.9 ± 11.9 | 0.059 |
| 51I/59R/108N/164L | 1.42 ±0.57 | 20.7 ± 6.8 | 37.3 ± 13.2 | 0.069 |
| 51I/59R/108N/213A | 0.68 ± 0.17 | 10.2 ± 5.1 | 30.9 ± 12.2 | 0.066 |
