Table 1.
AFC and apoptosis values for cells treated with GSNO with and without the caspase 3 inhibitor DEVD-CHO
| Cell | Treatment | AFC μM | Apoptosis % |
|---|---|---|---|
| RAW | Control | 0.043 ± 0.02 | 1.9 ± 0.3 |
| GSNO (200 μM) | 0.21 ± 0.13 | 7.5 ± 1.4 | |
| GSNO (200 μM) + DEVD-CHO (10 μM) | 0.08 ± 0.04 | 5.8 ± 1.2 | |
| GSNO (1 mM) | 0.92 ± 0.32 | 14.2 ± 2.6 | |
| GSNO (1 mM) + DEVD-CHO (10 μM) | 0.08 ± 0.06 | 9.9 ± 2.4 | |
| RES | Controlc | 0.087 ± 0.01 | 1.7 ± 0.4 |
| GSNO (200 μM) | 1.32 ± 0.33 | 15.7 ± 3.6 | |
| GSNO (200 μM) + DEVD-CHO (10 μM) | 0.06 ± 0.04 | 12.5 ± 1.3 | |
| GSNO (1 mM) | 1.13 ± 0.01 | 19.2 ± 3.4 | |
| GSNO (1 mM) + DEVD-CHO (10 μM) | 0.07 ± 0.01 | 15.6 ± 2.4 |
Apoptosis was calculated from intercalation of propidium iodide into DNA from ethanol-fixed RAW or RES cells that had been treated with the indicated amounts of GSNO and DEVD-CHO. Control values were derived from untreated cell populations.