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Giα1-mediated inhibition of endocytosis is partially reversed by C3 exoenzyme. CV1 cells infected with adenoviruses encoding Giα1 or β-galactosidase were scrape-loaded with C3 (10 μg/ml) 24 h after infection (32). 125I-LDL uptake was quantified 24 h after scrape loading, as described under Materials and Methods. Error bars represent standard deviations (n = 3).
