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. 1998 Apr 28;95(9):5061–5066. doi: 10.1073/pnas.95.9.5061

Figure 3.

Figure 3

Effect of inhibitors on HOCl-mediated APE-1 mRNA induction. (A) After treatment with 130 nM HOCl for 9 h, aliquots of HeLa cells were exposed to no drug (lane 2), 5 μg/ml actinomycin D (lane 3), or 100 μg/ml anisomycin (lane 4) for 180 min before extraction of RNA, which was then used for the Northern blot analysis. Lane 1, APE-1 mRNA from control (without HOCl treatment) cells. (B) Abrogation of APE-1 activation by protein kinase inhibitor or ROS scavenger. Logarithmic-phase HeLa cells were mock-treated (lane 1) or treated with 65 nM HOCl (lane 2), 130 nM HOCl (lane 3), simultaneously with 130 nM HOCl and 20 mM N-acetylcysteine (lane 4), or 10 nM staurosporine (lane 5), or 20 nM H7 (lane 6). Northern blot analysis was performed after 12 h of treatment. The blots were reprobed for 18S ribosomal RNA (Lower) as internal control as in Fig. 1.