Figure 8.
Lack of APE-1 activation by alkylating agents and UV light. Logarithmic-phase HeLa cells were mock-treated (lane 1), treated with 2 mM MMS (lane 2) or 50 μM N,N′-bis(2-chloroethyl)-N-nitrosourea (lane 3), or exposed to 254-nm UV light at 10 J/m2 (lane 4). Cells were incubated further for 9 h and harvested for Northern blot analysis. The blot was reprobed with 18S ribosomal RNA (Lower).