Table 2.
Genotype | Percent animals reaching adult after 4 days at 20°C* | n |
---|---|---|
Wild type (N2) | 98 | 305 |
ceh-22(cc8266) | 36 | 230 |
ceh-22(cc8266); cuEx(ceh-22∷nkx2.5 cDNA) | 68 | 104 |
ceh-22(cc8266); cuEx(ceh-22∷ceh-22 cDNA) | 77 | 203 |
Embryos segregating from the indicated strains were picked to fresh plates and incubated at 20°C. After 4 days, the number of animals reaching adulthood was determined. Neither ceh-22∷ceh-22 nor ceh-22∷nkx2.5 rescued a partially penetrant sterile phenotype exhibited by ceh-22(cc8266) (ref. 15; data not shown). This phenotype is rescued by transformation with a ceh-22 genomic DNA fragment; thus, we believe the ceh-22∷ceh-22 and ceh-22∷nkx2.5 may be missing sequences necessary for rescue of sterility.