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Transplantation of engrafted fat pads. Whole-mount preparations of transplanted reconstituted breasts. Fat pads from PR−/− or PR+/+ mice were engrafted with ROSA26 (β-galactosidase+) PR+/+ primary mammary epithelial cells and transplanted onto the abdominal muscle wall of PR+/+.RAG1−/− recipients. the reconstituted mammary glands were removed from the recipients after parturition and stained with X-Gal before whole-mounting.
