Figure 1.

Targeted disruption of FGFR2. (A) Genomic map and its manipulation. Above the line: H, HindIII; C, ClaI. Under the line: C, KV, and BV, exons 7, 8, and 9 (IIIc loop); TM, exon 10 (transmembrane); K1, kinase domain 1; ATP, exon 12 encoding the ATP binding site; neo, neomycin resistance gene. (B) Map of the recombinant, flanking sequences blocked, abbreviations as in A. (C) Wild-type (6.2-kb) and recombinant (9.4-kb) HindIII fragments. (D) Southern analysis of recombinant ES cells. Clones shown in lanes 1 and 2 were used for aggregation. The recombinant allele was analyzed also by a 3′ probe demonstrating the large deletion and the absence of the EcoRV site of exon 9. Specific probes showed the absence of the transmembrane domain.