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. 1993 Mar;59(3):851–859. doi: 10.1128/aem.59.3.851-859.1993

Characterization of Xanthomonas campestris Pathovars by rRNA Gene Restriction Patterns

Yvette Berthier 1,*, Valérie Verdier 2, Jean-Luc Guesdon 3, Danièle Chevrier 3, Jean-Baptiste Denis 1, Guy Decoux 1, Monique Lemattre 1
PMCID: PMC202199  PMID: 16348894

Abstract

Genomic DNA of 191 strains of the family Pseudomonadaceae, including 187 strains of the genus Xanthomonas, was cleaved by EcoRI endonuclease. After hybridization of Southern transfer blots with 2-acetylamino-fluorene-labelled Escherichia coli 16+23S rRNA probe, 27 different patterns were obtained. The strains are clearly distinguishable at the genus, species, and pathovar levels. The variability of the rRNA gene restriction patterns was determined for four pathovars of Xanthomonas campestris species. The 16 strains of X. campestris pv. begoniae analyzed gave only one pattern. The variability of rRNA gene restriction patterns of X. campestris pv. manihotis strains could be related to ecotypes. In contrast, the variability of patterns observed for X. campestris pv. malvacearum was not correlated with pathogenicity or with the geographical origins of the strains. The highest degree of variability of DNA fingerprints was observed within X. campestris pv. dieffenbachiae, which is pathogenic to several hosts of the Araceae family. In this case, variability was related to both host plant and pathogenicity.

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Selected References

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  1. Bingen E. H., Denamur E., Lambert-Zechovsky N. Y., Bourdois A., Mariani-Kurkdjian P., Cezard J. P., Navarro J., Elion J. DNA restriction fragment length polymorphism differentiates crossed from independent infections in nosocomial Xanthomonas maltophilia bacteremia. J Clin Microbiol. 1991 Jul;29(7):1348–1350. doi: 10.1128/jcm.29.7.1348-1350.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boucher C. A., Van Gijsegem F., Barberis P. A., Arlat M., Zischek C. Pseudomonas solanacearum genes controlling both pathogenicity on tomato and hypersensitivity on tobacco are clustered. J Bacteriol. 1987 Dec;169(12):5626–5632. doi: 10.1128/jb.169.12.5626-5632.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boucher C. A., Van Gijsegem F., Barberis P. A., Arlat M., Zischek C. Pseudomonas solanacearum genes controlling both pathogenicity on tomato and hypersensitivity on tobacco are clustered. J Bacteriol. 1987 Dec;169(12):5626–5632. doi: 10.1128/jb.169.12.5626-5632.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Grimont F., Chevrier D., Grimont P. A., Lefevre M., Guesdon J. L. Acetylaminofluorene-labelled ribosomal RNA for use in molecular epidemiology and taxonomy. Res Microbiol. 1989 Sep;140(7):447–454. doi: 10.1016/0923-2508(89)90065-x. [DOI] [PubMed] [Google Scholar]
  5. Grimont F., Grimont P. A. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur Microbiol. 1986 Sep-Oct;137B(2):165–175. doi: 10.1016/s0769-2609(86)80105-3. [DOI] [PubMed] [Google Scholar]
  6. Guesdon J. L. Immunoenzymatic techniques applied to the specific detection of nucleic acids. A review. J Immunol Methods. 1992 Jun 24;150(1-2):33–49. doi: 10.1016/0022-1759(92)90063-y. [DOI] [PubMed] [Google Scholar]
  7. Koblavi S., Grimont F., Grimont P. A. Clonal diversity of Vibrio cholerae O1 evidenced by rRNA gene restriction patterns. Res Microbiol. 1990 Jul-Aug;141(6):645–657. doi: 10.1016/0923-2508(90)90059-y. [DOI] [PubMed] [Google Scholar]
  8. Masse M. J., Meulien P., Le Guern A., Kourilsky P. Monoclonal antibody detection of 2-acetyl-aminofluorene-modified DNA probes for the specific detection of nucleic acids in hybridization procedures. Ann Inst Pasteur Immunol. 1985 Nov-Dec;136D(3):231–243. doi: 10.1016/s0769-2625(85)80109-4. [DOI] [PubMed] [Google Scholar]
  9. Pérolat P., Grimont F., Regnault B., Grimont P. A., Fournié E., Thevenet H., Baranton G. rRNA gene restriction patterns of Leptospira: a molecular typing system. Res Microbiol. 1990 Feb;141(2):159–171. doi: 10.1016/0923-2508(90)90025-l. [DOI] [PubMed] [Google Scholar]
  10. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  11. Van den Mooter M., Swings J. Numerical analysis of 295 phenotypic features of 266 Xanthomonas strains and related strains and an improved taxonomy of the genus. Int J Syst Bacteriol. 1990 Oct;40(4):348–369. doi: 10.1099/00207713-40-4-348. [DOI] [PubMed] [Google Scholar]

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