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. 1998 Apr 28;95(9):5121–5126. doi: 10.1073/pnas.95.9.5121

Figure 1.

Figure 1

Schematic representation of the zebrafish GATA-2 genomic locus before and after modification by the Chi-containing G2TC DNA fragment. (A) Genomic structure of the three GATA-2 BAC clones and their relationships to the ATG initiation codon. Arrows indicate the orientation of the GATA-2 gene. (B) Region of the wild-type GATA-2 gene locus targeted for homologous recombination. (C) The nonreplicative G2TC DNA fragment containing Chi sites properly situated to promote homologous recombination (solid arrows). The edges of the 5′ and 3′ regions of homology with the GATA-2 gene locus are indicated by dashed lines. (D) GATA-2 gene locus after replacement of the first coding exon with the GFP and kanamycin genes. This modification adds several restriction sites to the GATA-2 gene as follows: B, BamHI; C, ClaI; Rv, EcoRV; H, HindIII; N, NotI; Sc, SacI; S, SalI; X, XhoI. Primers P1/P2 and P3/P4 were used in a PCR to identify BAC DNA with the correct gene replacement. Primers P1 and P4 were used to sequence the edges of the 5′ and 3′ regions of homology, respectively.