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. 1998 Apr 28;95(9):5133–5138. doi: 10.1073/pnas.95.9.5133

Figure 1.

Figure 1

RT-PCR analysis of XIST and MIC2 (escapes X inactivation) expression in clones derived from the active X hybrid, GM06318, after 5aCdr treatment. Unselected clones of 5aCdr-treated GM06318 were examined for XIST reactivation at an early stage of growth by RT-PCR. Representative data are shown from a total of 48 clones analyzed that were positive for expression of MIC2, XIST, or both. Some clones were positive for XIST and not MIC2 because of a spurious problem with MIC2 amplification in duplex PCR at low template concentrations (data not shown). Control cells included GM06318 and the inactive X hybrid X8–6T2S1. Arrows point to clones scored as positive for XIST reactivation.