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. 1998 Apr 28;95(9):5133–5138. doi: 10.1073/pnas.95.9.5133

Figure 5.

Figure 5

FISH analysis of XIST RNA localization. To examine further the characteristics of the XIST RNA product in reactivant cells, its cellular location was examined by hybridization to a genomic cosmid clone containing XIST RNA sequences. Cells were fixed with 3:1 methanol to acetic acid and hybridized with biotin-labeled probe without prior denaturation of cellular DNA. (A) Normal human male fibroblasts. (B) Normal human female fibroblasts. (C) The XIST reactivant clone 4F5 derived from GM06318 after 5aCdr treatment. (D) The inactive X human–hamster hybrid 8121-TGRD. (E and F) XIST reactivant cells derived from a normal human male fibroblasts after 5aCdr treatment.