Table 1.
Gradient elution for three solvent systems for HPLC.
| Solvent system (%)
|
||||
|---|---|---|---|---|
| Time (min) | Flow (mL/min) | A | B | C |
| 0.00 | 0.3 | 60 | 25 | 15 |
| 5.00 | 0.3 | 50 | 25 | 25 |
| 6.00 | 0.3 | 30 | 25 | 45 |
| 15.00 | 0.3 | 30 | 25 | 45 |
| 16.00 | 0.3 | 60 | 25 | 15 |
| 20.00 | 0.3 | 60 | 25 | 15 |
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