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. 1998 Apr 28;95(9):5145–5149. doi: 10.1073/pnas.95.9.5145

Figure 3.

Figure 3

(A) Linking of SpeI fragments of symbiotic element DNA by hybridization. R12C and R16C are diverse symbiotic strains that received the element from ICMP3153 in the field (17). Cosmids used for linking the fragments are named below the panels and are indicated together with other cosmids used for walking above the map in B. (B) Physical map of the element and location of features identified by sequencing of ICMP3153 DNA. Sizes of SpeI (S) fragments are shown in kilobases. There is 1.6 kb between the left junction of the element and the first SpeI site and 52 kb between the last SpeI site and the right junction. The sizes of the sequenced regions from ICMP3153 at the left junction (GenBank accession no. AF049242) and right junction (AF049243) are shown in base pairs. The region upstream of the element showed homology to the Escherichia coli melibiose regulatory gene melR (U14003), and the sugar-binding protein gene lacE from Agrobacterium radiobacter (X66596). The sequence at the right end homologous to fmet-tRNA is shown in comparison to sequence from Bartonella bacilliformis (L10238).