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. 1998 Apr 28;95(9):5150–5155. doi: 10.1073/pnas.95.9.5150

Figure 3.

Figure 3

(A) Southern blot analysis of DNA digestion with BglII and hybridization with the SOU1 probe of the following strains: CAF4–2, the normal SOU1/SOU1 strain; CA18, the singly disrupted SOU1/sou1-Δ∷hisG-URA3-hisG strain; CA19, the singly disrupted SOU1/sou1-Δ∷hisG strain; CA20, the doubly disrupted sou1-Δ∷hisG/sou1-Δ∷hisG-URA3-hisG strain; and CA21, the doubly disrupted sou1-Δ∷hisG/sou1-Δ∷hisG strain. (B) Comparative growth of CAF4–2 and CA19 and CA21 on glucose medium (SD plus uridine) for 6 days at 37°C and (C) on l-sorbose medium (plus uridine) for 14 days at 37°C. Each strain was spotted as serial 1/10 dilutions, from left to right, starting from an initial concentration of 106 cells per ml.