Figure 2.

Order of testing does not affect impairments in memory for contextual conditioning as a result of 0–5 h of total sleep deprivation after training. Mice were trained similarly to the experiment shown in Figure 1, however, freezing in response to the conditioned stimulus (CS) in an altered context was tested at 24 h following training before freezing in response to the shocked context was tested. (A) Mice sleep deprived from 0–5 h after training freeze less in response to the shocked context than do nonsleep-deprived mice (context; P < .05; n = 9 per group). Sleep-deprived mice did not significantly differ from nonsleep-deprived mice in freezing in the altered chamber (pre CS) or in freezing in response to the cue (CS; P's > .05; n = 9 per group). (B) Mice show a deficit in the specificity of freezing to the shocked context when sleep deprived from 0–5 h after training. Specificity of freezing to the shocked context (delta context) measured by the difference in percent freezing between the shocked and altered context was significantly less in mice that were sleep deprived from 0–5 h after training than in nonsleep-deprived mice (P < .01; n = 9 per group). Cue-specific freezing (delta cue) is not altered by sleep deprivation from 0–5 h after training (P > .05; n = 15 per group). *, P < .05.