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DSB repair substrate DRneo. (A) The DRneo substrate contains the 18-bp cleavage site for the rare-cutting endonuclease I-SceI (black bar) inserted in the S2neo gene. The homology between the S2neo gene and the 685-bp 3′ neo gene is indicated by the stippling. (B) Two outcomes of DSB-induced homologous recombination that produce a neo+ gene.
